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About Me

Curriculum Vitae

Professional Achievements

15 Years in Life Science

4 Key Principles


Advanced Data Analytics
New Regulatory Pathways
Leading Key Publications
Drug-, CDx-, IVD- and Biomarker-Deverlopment


Founder of the IMI SAFE-T Consortium
First Successful Biomarker Qualification
Innovation Through In-licensing
Collaboration with BMGF


Translational Medicine and Personalized Medicine
Developing Companion Diagnostics Tests
Development of Niji PoC IVD Platform
Launch of Niji and Total IgE IVD Tests


Acquisition of the Biotech Vivacta
Building-up the Novartis Kent Site
Leading the Change Management of NPT

Ph. D. Thesis


Abstract in German (Zusammenfassung)

Table of Contents

1. Introduction

1.1. Outline

2. Theory Fundamentals of the Multivariate Data Analysis

2.1. Overview of the Multivariate Quantitative Data Analysis

2.2. Experimental Design

2.3. Data Preprocessing

2.4. Data Splitting and Validation

2.4.1. Crossvalidation
2.4.2. Bootstrapping
2.4.3. Random Subsampling
2.4.4. Kennard Stones
2.4.5. Kohonen Neural Networks
2.4.6. Conclusions

2.5. Calibration of Linear Relationships

2.5.1. PLS

2.6. Calibration of Nonlinear Relationships

2.7. Neural Networks Universal Calibration Tools

2.7.1. Principles of Neural Networks
2.7.2. Topology of Neural Networks
2.7.3. Training of Neural Networks

2.8. Too Much Information Deteriorates Calibration

2.8.1. Overfitting, Underfitting and Model Complexity
2.8.2. Neural Networks and the Complexity Problem
2.8.3. Brute Force Variable Selection
2.8.4. Variable Selection by Stepwise Algorithms
2.8.5. Variable Selection by Genetic Algorithms
2.8.6. Variable Selection by Simulated Annealing
2.8.7. Variable Compression by Principal Component Analysis
2.8.8. Topology Optimization by Pruning Algorithms
2.8.9. Topology Optimization by Genetic Algorithms
2.8.10. Topology Optimization by Growing Neural Network Algorithms

2.9. Measures of Error and Validation

3. Theory Quantification of the Refrigerants R22 and R134a: Part I

3.1. Experimental

3.2. Single Analytes

3.3. Sensitivities

3.4. Calibrations of the Mixtures

3.5. Variable Selection by Brute Force

3.6. Conclusions

4. Experiments, Setups and Data Sets

4.1. The Sensor Principle

4.2. SPR Setup

4.3. RIfS Sensor Array

4.4. 4l Miniaturized RIfS Sensor

4.5. Data Sets

4.5.1. Refrigerants R22 and R134a R22 and R134a by the SPR Setup R22 and R134a by the RIfS Array and the 4l-Setup
4.5.2. Homologous Series of the Low Alcohols Methanol and Ethanol by the SPR Setup Methanol, Ethanol and Propanol by the SPR Setup Methanol, Ethanol and Propanol by the RIfS Array and the 4l Setup Quaternary Mixtures of Alcohols by the RIfS Array and the SPR Setup

5. Results Kinetic Measurements

5.1. Static Sensor Measurements

5.2. Time-resolved Sensor Measurements

5.3. Makrolon A Polymer for Time-resolved Measurements

5.3.1. General Properties of Makrolon
5.3.2. Time-resolved Measurements
5.3.3. Thickness of the Sensitive Layer
5.3.4. Influence of the Carrier Gas

5.4. Conclusions

6. Results Multivariate Calibrations

6.1. PLS Calibration

6.1.1. Wald-Wolfowitz Runs Test
6.1.2. Durbin-Watson Statistics
6.1.3. Results of Statistical Tests

6.2. Box-Cox Transformation + PLS

6.3. INLR

6.4. QPLS

6.5. CART

6.6. Model Trees

6.7. MARS

6.8. Neural Networks

6.9. PCA-NN

6.10. Neural Networks and Pruning

6.11. Conclusions

7. Results Genetic Algorithm Framework

7.1. Single Run Genetic Algorithm

7.2. Genetic Algorithm Framework - Theory

7.3. Genetic Algorithm Framework - Results

7.4. Genetic Algorithm Framework Conclusions

8. Results Growing Neural Network Framework

8.1. Modifications of the Growing Neural Network Algorithm

8.2. Application of the Growing Neural Networks

8.3. Growing Neural Network Algorithm Frameworks

8.4. Applications of the Growing Neural Network Frameworks

8.4.1. Parallel Framework
8.4.2. Loop-based Framework

8.5. Conclusions and Comparison of the Different Methods

9. Results All Data Sets

9.1. Methanol and Ethanol by SPR

9.1.1. Single Analytes
9.1.2. Parallel Growing Neural Network Framework
9.1.3. Sensitivity Analysis
9.1.4. Brute Force Variable Selection
9.1.5. Conclusions

9.2. Methanol, Ethanol and 1-Propanol by SPR

9.2.1. Single Analytes
9.2.2. Multivariate Calibrations of the Mixtures
9.2.3. Genetic Algorithm Framework
9.2.4. Parallel Growing Neural Network Framework
9.2.5. PCA-NN
9.2.6. Conclusions

9.3. Methanol, Ethanol and 1-Propanol by the RIfS Array and the 4l Setup

9.3.1. Signals and Data Preparation
9.3.2. Mixtures by the RIfS Array
9.3.3. Mixtures by the 4l Setup
9.3.4. Conclusions

9.4. Quaternary Mixtures by the SPR Setup and the RIfS Array

9.4.1. Introduction
9.4.2. Results
9.4.3. Conclusions

9.5. Quantification of the Refrigerants R22 and R134a in Mixtures: Part II

10. Results Various Aspects of the Frameworks and Measurements

10.1. Single or Multiple Analyte Rankings

10.2. Stopping Criteria for the Parallel Frameworks

10.3. Optimization of the Measurements

10.4. Robustness and Comparison with Martens' Uncertainty Test

11. Summary and Outlook

12. References

13. Acknowledgements


Publications and Patents

Poster Gallery

Research Tutorials


Biology and "-omics"

Definition of Metabonomics


In-life phase
Animal Study
Human Study
Analytical Techniques
Structure Elucidation
Spectral Exclusion Regions
Peak Shifts
Equidistant Binning
Non-equidistant Binning
Probabilistic Normalization
Data Analysis
Principles of PCA
PCA in Metabonomics
Metabolite Projection Analysis
Biochemical Interpretation

Applications in Drug Development

Possiblities and Examples
Specific Biomarkers
PAG as Biomarker for Phospholipidosis
Amiodarone Study
Monitoring Drug Candidates
Patterns of Biomarkers
Competitive Study
Compound A
Compound B
Interpretations for Compound B
Predictions and Results
Gut Microflora
Normal Gut Microflora
Abnormal Gut Microflora
Structure Elucidation of New Biomarkers
Competitive Study
Metabolite Enrichement
Biochemical Interpretation
Ranking Study
Influences of Study Protocol
Clustering of Compounds
Statistical Identification of Correlated Signals
Validation of Structure Identity
Biochemical Interpretation


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